- Product Details
- Specifications
- Data /
Performance - Product Resources
The PHASIFY™ DeCOVID SARS-CoV-2 RT-qPCR Kit is a RT-qPCR kit for detecting SARS-CoV-2 from human respiratory specimens, including nasopharyngeal swabs, oropharyngeal swabs and sputum. Developed in collaboration with Prof. Leo Poon, one of the world's leading scientists in emerging viruses at a Hong Kong University, the PHASIFY™ DeCOVID kit is a multiplex RT-qPCR detecting two specific sequences in the ORF1b region of SARS-COV-2 in a single reaction together with human RNase P housekeeping gene as the internal control
PHASIFY™ DeCOVID Advantage
Product Details
| Product Code | Unit Size |
|---|---|
| 3010100 | 100x reactions |
Specifications
| Features | Specifications |
|---|---|
| Test Principle | One-step reverse transcription real time PCR |
| Targets |
Orf1b and RdRP as viral target, RNase P as human internal control |
| Specimen type | Respiratory specimens including nasopharyngeal swab, oropharyngeal swab, and sputum |
| Input volume | 10 µL |
| Detection instrument |
Real time PCR instrument with FAM, VIC and TAMRA detection channels |
| Limit of detection |
10 viral copies / mL (when used with the PHASIFY™ VIRAL RNA Extraction Kit) |
| Apporopriate run time (per 60 preps) | 2 hours |
| Shelf life | 6 months |
| Intended use | For qualitative detection of SARS-CoV-2 nucleic acid extracted from human respiratory clinical specimens |
| Processing | Manual |
| Shipping Condition | Cold storage (dry ice) |
| Storage condition | -20ºC or below, avoid repeated freeze thaw cycles |
Data / Performance
PHASIFY™ DeCOVID demonstrated superior performance in detecting ORF-1b targets when compared to BGI’s FDA EUA RT-PCR Kit. When PHASIFY™ DeCOVID is combined with our PHASIFY™ VIRAL RNA extraction kit, the limit of detection of SARS-CoV-2 is further improved to as low as 10 cp / mL
I. Reduced Ct value against industry leading brand (BGI)
Figure 1 - SARS-CoV-2 virus in swab was extracted in the concentrations of 200, 500, 1000 and 5000 cp / mL using Qiagen Viral Mini RNA extraction kit. The virus RNA was amplified and detected using the multiplex PHASIFY™ DeCOVID SARS-CoV-2 RT-qPCR Kit or BGI qPCR Kit respectively. LoD for PHASIFY™ DeCOVID and BGI is 200 cp / mL and 500 cp / mL in swab specimen respectively
Figure 1 - SARS-CoV-2 virus in swab was extracted in the concentrations of 200, 500, 1000 and 5000 cp / mL using Qiagen Viral Mini RNA extraction kit. The virus RNA was amplified and detected using the multiplex PHASIFY™ DeCOVID SARS-CoV-2 RT-qPCR Kit or BGI qPCR Kit respectively. LoD for PHASIFY™ DeCOVID and BGI is 200 cp / mL and 500 cp / mL in swab specimen respectively
II. Increased screening sensitivity with the use of PHASIFY™ VIRAL
Figure 2 - SARS-CoV-2 virus in swab was extracted in the concentrations of 10, 20, 50, 100 and 200 cp / mL using PHASIFY™ VIRAL RNA extraction kit. The virus RNA was amplified and detected using the multiplex PHASIFY™ DeCOVID SARS-CoV-2 RT-qPCR Kit. LoD is 10 cp /mL in oropharyngeal swab specimen.
Figure 2 - SARS-CoV-2 virus in swab was extracted in the concentrations of 10, 20, 50, 100 and 200 cp / mL using PHASIFY™ VIRAL RNA extraction kit. The virus RNA was amplified and detected using the multiplex PHASIFY™ DeCOVID SARS-CoV-2 RT-qPCR Kit. LoD is 10 cp /mL in oropharyngeal swab specimen.
Product Resources
| User Manual | |
| Instruction for Use | |
| Product Catalogue | |
| Safety Data Sheets (SDS) |
a. SDS Nuclease free water b. SDS 2x Reaction Buffer c. SDS Enzyme Mix d. SDS Primer Probe Mix e. SDS No Template Control f. SDS Positive Template |
Product Sales and Customer Services
| Email : phasify@phasesci.com | | | Customer Service : | Hong Kong | +852 3892-7200 (9am - 6pm UTC+8) |
| US | +1 (657) 233-5880 (9am - 6pm Pacific) | |||
| Technical Support : | Hong Kong | +852 9135-2170 (9am - 6pm UTC+8) | ||
| US | +1 (657) 296-6106 (9am - 6pm Pacific) |
| Customer Service : |
Hong Kong +852 3892-7200
(9am - 6pm UTC+8)
US +1 (657) 233-5880
(9am - 6pm Pacific) |
| Technical Support : |
Hong Kong +852 9135-2170
(9am - 6pm UTC+8)
+1 (657) 296-6106
(9am - 6pm Pacific) |
