- Product Details
- Specifications
- Data /
Performance - Product Resources
The PHASIFY™ ENRICH cfDNA Extraction Kit is designed for maximum yield of cfDNA from plasma and superior enrichment of cfDNA by size selection.
PHASIFY™ ENRICH Advantage
Product Details
| Product Code | Input Volume | Unit Size |
|---|---|---|
| 1020100 | 1 mL plasma | 25x reactions |
| 1020200 | 2 mL plasma | 25x reactions |
Specifications
| Features | Specifications |
|---|---|
| Applications | ddPCR, PCR, real-time PCR, NGS, etc. |
| Sample type | Plasma |
| Appropriate run time per 20 preps | ~210 mins |
| Elution volume | 5 µL - 150 µL |
| Shelf life stability | 12 months |
| Intended use | Research Use Only (RUO) |
| Processing | Manual |
| Shipping Condition | Room Temperature |
| Storage Condition |
RT Box: Stored at 15-30 °C Cold Box: Stored at 4°C or below |
Data / Performance
The PHASIFY™ ENRICH cfDNA Extraction Kit, powered by novel PHASIFY™ technology, demonstrated 2.5-fold greater yields of circulating cell-free tumor DNA (ctDNA) in cancer patient plasma compared to the Qiagen QIAamp Circulating Nucleic Acid kit
I. Improved yield of circulating tumor DNA (ctDNA)
Figure 1 - The cfDNA was extracted from 28 tissue-positive cancer patient plasma samples (1 mL) using PHASIFY™ ENRICH and the QIAamp CNA kit. On average PHASIFY™ ENRICH demonstrated a 2.5-fold increase in mutant copy recovery compared to QIAamp CNA (P-Value <0.01 Wilcoxon Signed-Rank).
Figure 1 - The cfDNA was extracted from 28 tissue-positive cancer patient plasma samples (1 mL) using PHASIFY™ ENRICH and the QIAamp CNA kit. On average PHASIFY™ ENRICH demonstrated a 2.5-fold increase in mutant copy recovery compared to QIAamp CNA (P-Value <0.01 Wilcoxon Signed-Rank).
II. Enrichment of cfDNA fragments and removal of contaminating genomic DNA
Figure 2 - DNA was extracted from 2 mL healthy human plasma spiked with 100 bp ladder using PHASIFY™ MAX and PHASIFY™ ENRICH. Fragment analyzer trace demonstrates significant removal of fragments above 500 bp while maintaining recovery of target cfDNA.
Product Resources
| Quick Start Protocol |
a. PHASIFY™ ENRICH cfDNA Extraction Kit Quick Start Protocol (1mL plasma input) b. PHASIFY™ ENRICH cfDNA Extraction Kit Quick Start Protocol (2 mL plasma input) |
| Product Catalogue | PHASIFY™ ENRICH cfDNA Extraction Kit Product Catalogue |
| Safety Data Sheets (SDS) |
a. SDS Solution A2 b. SDS Solution B c. SDS Solution C d. Solution P e. SDS D1 f. SDS Buffer D2 g. SDS A1 h. SDS Solution D3 |
Product Sales and Customer Services
| Email : phasify@phasesci.com | | | Contact : | Hong Kong | +852 3892-7211 (9am - 6pm UTC+8) |
| US | +1 (657) 233-5880 (9am - 6pm Pacific) |
| Contact : |
Hong Kong +(852) 3892-7211
(9am - 6pm UTC+8)
US +1 (657) 233-5880
(9am - 6pm Pacific) |
