• Product Details
  • Specifications
  • Data /
    Performance
  • Product Resources

The PHASIFY™ ENRICH cfDNA Extraction Kit is designed for maximum yield of cfDNA from plasma and superior enrichment of cfDNA by size selection.

PHASIFY™ ENRICH Advantage

High Yield and Concentration: Multiple-fold increase in cell-free DNA recovery with flexible elution volume
High Purification: Removal of contaminating genomic DNA, without loss to target cfDNA
Enrichment by Size Selection: Removal of >500 bp high molecular weight DNA
Modifiable Size Selection: Contact us to discuss custom solutions

Product Details

Product Code Input Volume Unit Size
1020100 1 mL plasma 25x reactions
1020200 2 mL plasma 25x reactions

Specifications

Features Specifications
Applications ddPCR, PCR, real-time PCR, NGS, etc.
Sample type Plasma
Appropriate run time per 20 preps ~210 mins
Elution volume 5 µL - 150 µL
Shelf life stability 12 months
Intended use Research Use Only (RUO)
Processing Manual
Shipping Condition Room Temperature
Storage Condition RT Box: Stored at 15-30 °C
Cold Box: Stored at 4°C or below

Data / Performance

The PHASIFY™ ENRICH cfDNA Extraction Kit, powered by novel PHASIFY™ technology, demonstrated 2.5-fold greater yields of circulating cell-free tumor DNA (ctDNA) in cancer patient plasma compared to the Qiagen QIAamp Circulating Nucleic Acid kit

I. Improved yield of circulating tumor DNA (ctDNA)

Figure 1 - The cfDNA was extracted from 28 tissue-positive cancer patient plasma samples (1 mL) using PHASIFY™ ENRICH and the QIAamp CNA kit. On average PHASIFY™ ENRICH demonstrated a 2.5-fold increase in mutant copy recovery compared to QIAamp CNA (P-Value <0.01 Wilcoxon Signed-Rank).

Figure 1 - The cfDNA was extracted from 28 tissue-positive cancer patient plasma samples (1 mL) using PHASIFY™ ENRICH and the QIAamp CNA kit. On average PHASIFY™ ENRICH demonstrated a 2.5-fold increase in mutant copy recovery compared to QIAamp CNA (P-Value <0.01 Wilcoxon Signed-Rank).

II. Enrichment of cfDNA fragments and removal of contaminating genomic DNA

Figure 2 - DNA was extracted from 2 mL healthy human plasma spiked with 100 bp ladder using PHASIFY™ MAX and PHASIFY™ ENRICH. Fragment analyzer trace demonstrates significant removal of fragments above 500 bp while maintaining recovery of target cfDNA.

Product Sales and Customer Services

Email : phasify@phasesci.com | Contact : Hong Kong +852 3892-7211 (9am - 6pm UTC+8)
US +1 (657) 233-5880 (9am - 6pm Pacific)
Email : phasify@phasesci.com
Contact :
Hong Kong +(852) 3892-7211
(9am - 6pm UTC+8)
US +1 (657) 233-5880
(9am - 6pm Pacific)