- 產品詳情
- 產品規格
- 數據/
產品表現 - 產品資料
The PHASIFY™ ENRICH cfDNA Extraction Kit is designed for maximum yield of cfDNA from plasma and superior enrichment of cfDNA by size selection.
PHASIFY™ ENRICH Advantage
產品詳情
| Product Code | Input Volume | Unit Size |
|---|---|---|
| 1020100 | 1 mL plasma | 25x reactions |
| 1020200 | 2 mL plasma | 25x reactions |
Specifications
| 功能 | 產品規格 |
|---|---|
| 應用 | ddPCR, PCR, real-time PCR, NGS, etc. |
| 樣本類型 | Plasma |
| 每20個測試大致操作用時 | ~210 mins |
| 洗脫體積 | 5 µL - 150 µL |
| 有效期 | 12 months |
| 使用範圍 | 僅供科研使用 |
| 操作 | 手工操作 |
| 運輸要求 | 室溫 |
| 儲存要求 |
RT Box: Stored at 15-30 °C Cold Box: Stored at 4°C or below |
數據/產品表現
The PHASIFY™ ENRICH cfDNA Extraction Kit, powered by novel PHASIFY™ technology, demonstrated 2.5-fold greater yields of circulating cell-free tumor DNA (ctDNA) in cancer patient plasma compared to the Qiagen QIAamp Circulating Nucleic Acid kit
I. Improved yield of circulating tumor DNA (ctDNA)
Figure 1 - The cfDNA was extracted from 28 tissue-positive cancer patient plasma samples (1 mL) using PHASIFY™ ENRICH and the QIAamp CNA kit. On average PHASIFY™ ENRICH demonstrated a 2.5-fold increase in mutant copy recovery compared to QIAamp CNA (P-Value <0.01 Wilcoxon Signed-Rank).
Figure 1 - The cfDNA was extracted from 28 tissue-positive cancer patient plasma samples (1 mL) using PHASIFY™ ENRICH and the QIAamp CNA kit. On average PHASIFY™ ENRICH demonstrated a 2.5-fold increase in mutant copy recovery compared to QIAamp CNA (P-Value <0.01 Wilcoxon Signed-Rank).
II. Enrichment of cfDNA fragments and removal of contaminating genomic DNA
Figure 2 - DNA was extracted from 2 mL healthy human plasma spiked with 100 bp ladder using PHASIFY™ MAX and PHASIFY™ ENRICH. Fragment analyzer trace demonstrates significant removal of fragments above 500 bp while maintaining recovery of target cfDNA.
產品資料
| 快速操作指南 |
a. PHASIFY™ ENRICH cfDNA Extraction Kit Quick Start Protocol (1mL plasma input) b. PHASIFY™ ENRICH cfDNA Extraction Kit Quick Start Protocol (2 mL plasma input) |
| Product Catalogue | PHASIFY™ ENRICH cfDNA Extraction Kit Product Catalogue |
| 安全數據表(SDS) |
a. SDS Solution A2 b. SDS Solution B c. SDS Solution C d. Solution P e. SDS D1 f. SDS Buffer D2 g. SDS A1 h. SDS Solution D3 |
客戶服務
| 電郵：phasify@phasesci.com | | | 聯繫方式： | 香港 | +852 3892-7211 (9am - 6pm UTC+8) |
| 美國 | +1 (657) 233-5880 (9am - 6pm Pacific) |
| 聯繫方式： |
Hong Kong +(852) 3892-7211
(9am - 6pm UTC+8)
US +1 (657) 233-5880
(9am - 6pm Pacific) |
