• Product Details
  • Specifications
  • Data /
    Performance
  • Product Resources

The PHASIFY™ MAX cfDNA extraction kit is designed for maximum yield of cfDNA from plasma and has demonstrated superior recovery against solid phase extraction kits.

PHASIFY™ MAX Advantages

High Yield: Multiple fold increase in cell-free DNA recovery compared to solid phase extraction
High Concentration: Flexible elution volume as low as 5 µL
Easy-to-Use: No specialized equipment needed such as magnetic racks and vacuum pumps

Product Details

Product Code Input Volume Unit Size
1010100 1 mL plasma 25x reactions
1010200 2 mL plasma 25x reactions

Specifications

Features Specifications
Applications ddPCR, PCR, real-time PCR, NGS, other
Sample type Plasma
Appropriate run time per 20 preps ~180 mins
Elution volume 5µL - 150µL
Shelf life stability 12 months
Intended use Research Use Only (RUO)
Processing Manual
Shipping Condition Room Temperature
Storage Condition RT Box: Stored at 15-30 °C
Cold Box: Stored at 4°C

Data / Performance

PHASIFY™ MAX cfDNA extraction kit, powered by novel PHASIFY™ technology, demonstrated 3-fold greater yields of circulating cell-free DNA (cfDNA) and circulating cell-free tumor DNA (ctDNA) in cancer patient plasma compared to the Qiagen QIAamp Circulating Nucleic Acid kit.

I. Improved yield of total DNA (cfDNA)

Figure 1 - The cfDNA was extracted from 89 tissue positive cancer patient plasma samples (1mL) using PHASIFY™ MAX and the QIAamp CNA kit. Total DNA recovery was measured using Quant-iT™ PicoGreen™ dsDNA Assay Kit. On average PHASIFY™ MAX demonstrated a 3.8-fold increase in total DNA recovery compared to QIAamp CNA (P-Value <0.0001 Wilcoxon Signed-Rank). Note, 2 outliers were excluded in which PHASIFY™ MAX generated 209-fold and 800-fold greater recovery.

Figure 1 - The cfDNA was extracted from 89 tissue positive cancer patient plasma samples (1mL) using PHASIFY™ MAX and the QIAamp CNA kit. Total DNA recovery was measured using Quant-iT™ PicoGreen™ dsDNA Assay Kit. On average PHASIFY™ MAX demonstrated a 3.8-fold increase in total DNA recovery compared to QIAamp CNA (P-Value <0.0001 Wilcoxon Signed-Rank). Note, 2 outliers were excluded in which PHASIFY™ MAX generated 209-fold and 800-fold greater recovery.

II. Improved mutation detection and clinical sensitivity

Tumor Type PHASIFY™ MAX (MAF) QIAamp CNA (MAF)
Pancreatic 0.35% ND
Pancreatic 0.65% ND
Pancreatic 0.41% ND
Appendiceal 0.35% ND
Ovarian 2.80% ND
Ovarian 0.44% ND
Colorectal 2.10% ND
Colorectal 0.24% ND
Colorectal 0.27% ND
Colorectal 0.25% ND
Colorectal 0.50% ND
Colorectal 0.35% ND
Colorectal 0.58% ND

ND = Not Detected, MAF = Mutant allele frequency

Figure 2 - In 13 samples, mutations were detected in samples extracted with PHASIFY™ MAX, but not with QIAamp CNA.

III. Greater recovery of desired cfDNA fragments

Figure 3 - DNA was extracted from 1 mL healthy human plasma using PHASIFY™ MAX or the QIAamp Circulating Nucleic Acid kit. The extracted DNA were analyzed on Agilent Bioanalyzer 2100, where 35bp and 10,380bp peaks correspond to internal markers.

Product Resources

User Manual PHASIFY™ MAX cfDNA Extraction Kit User Manual
Quick Start Protocol a. PHASIFY™ MAX cfDNA Extraction Kit Quick Start Protocol (1mL plasma input)
b.PHASIFY™ MAX cfDNA Extraction Kit Quick Start Protocol (2 mL plasma input)
Product Catalogue PHASIFY™ MAX cfDNA Extraction Kit Product Catalogue
Video a. PHASIFY™ MAX cfDNA Extraction Kit (1 mL plasma input)Demo Video
b. PHASIFY™ MAX cfDNA Extraction Kit (2 mL plasma input)Demo Video
Safety Data Sheets (SDS) a. SDS Solution A2
b. SDS Solution B
c. SDS Solution C
d. SDS D1
e. SDS Buffer D2
f. SDS A1
g. SDS Solution D3
FAQs a. PHASIFY™ MAX cfDNA Extraction Kit FAQs (1 mL plasma input)
b. PHASIFY™ MAX cfDNA Extraction Kit FAQs (2 mL plasma input)

Product Sales and Customer Services

Email : phasify@phasesci.com | Contact : Hong Kong +(852) 3892-7211 (9am - 6pm UTC+8)
US +1 (657) 233-5880 (9am - 6pm Pacific)
Email : phasify@phasesci.com
Contact :
Hong Kong +(852) 3892-7211
(9am - 6pm UTC+8)
US +1 (657) 233-5880
(9am - 6pm Pacific)